Chinese Journal of Physiology

MITOCHONDRIA-GOLGI COMPLEX 31

The nature of the changes exhibited by the red stained bodies during physiological activity lead us to suspect that they are closely associated with the Golgi material, while the fact that both neutral red and Sudan III are soluble in lipoid, and especially that lipoid will remove neutral red from water at once suggests that the Golgi material or some part of it is the substance coloured. This belief was further strengthened by the observation that both osmium and neutral redSudan III methods reveal the same changes, progressing at the same rate in the osmiophile and red material (Golgi lipoid) of one gland, whether pancreas or salivary (figs. 1-15 and 17-31). We have thus no hesitation in affirming that the neutral red-Sudan III method stains the Golgi material.

There is one point which must be emphasized. While in osmium preparations, the Golgi lipoid appears as a plexus or network, the neutral red-Sudan III stained preparation reveals chiefly droplets, a network being occasionally found. Whether or not any alteration of Golgi lipoid occurs after impregnation in the warm OsO, bath or whether the neutral red-Sudan III method stains only a part of the Golgi elements cannot be decided at present.

Mitochondria were again found in connection with the Golgi elements in the fresh material, the club-shaped mitochondria being not infrequently stained red at its enlarged end (fig. 34). This indicates, as we have pointed out before, that the Golgi lipoid is probably a product of mitochondrial dissociation, It should be added that the Golgi material (i.e. substances stainable by osmium, silver or neutral red-Sudan III method) is not only found at the ends but also in the body of mitochondria.

Lastly, we wish to draw attention to figs. 35-38. Concerning the fate of the Golgi material, it has been shown that, in the gastric glands, part is extruded (Ma, Lim and Liu, 25; Ma, 27). It has been abundantly demonstrated that Golgi material may be extruded by other glands, viz., mammary glands (Da Fano, 14; Beams, 4), sebaceous or oil glands (Bowen, 8), epithelia of oviduct (Brambell, 7). In the figures referred to it will be noted that as secretion progresses the Golgi material fragments and the fine particles are poured into the lumen along with a fine coagulate: We have found that osmiophile particles are present in the lumen of the ligatured pancreatic duct after stimulation of the gland, and that those particles behave like Golgi lipoid in being dissolved with difficulty by turpentine. They are perhaps more readily soluble