Chinese Journal of Physiology

ACID- AND BASE-BINDING POWER OF PROTEINS 23 TABLE 10,

Effect of denaturation and coagulation on base-binding power of serum albumin

20 cc of 0.55 per cent serum albumin solution-+graded amounts of N/10 HCl or N/10 NaOH.

a ee — —

| Before heating After heating No. | N/10 HCI | N/10 HCL | pH of pH or | pH of | N/20 NaCl! solution | difference |N/10 NaOH Pinion N/10 ‘NaOH added or from added | added suspensicn| control ce 7 ce cc |

1 | 2:0 HC | 2.388 2.4 NaOH 0.6 10,22 0,00

2 1.5 2.61 1.9 1.6 10.15 | —0.07

3 1.0 | | 14 2.6 10,15 | —0.07 + 0.8 1.2 3.0 10.16 —0.06 5 0.5 0.9 3.6 10.16 —0,06 6 0.3 0.7 4.0 10.18 —0.04 7 0.2 0.6 4.2 10.31 0.09 S* 0.1 5.64 | 0.5 44 10.36 0.14 y* 0.0 | O4 46 10,24 0,02 10* 0.1 NaOH 0.3 46 10,12 —0.10 Wd || -02 | | 0.2 46 10.01 —0.2] 12 | 0.4 | 10.22 | 0.0 4.6 9,77 —0.45 13 0.6 0.2 HCl 42 9.34 —0.88 14 0.8 0.94 0.4 3.8 8.97 =1:25

Unheated control | 0 4 46 10,22 |

Change in pH

a

16 1.0 0.5 0.0 0.5

cof Hol added-—r-4Co NaOH added

Fig, % Chavgein acid-binding power of serum albumin on denaturation aud coagulation.